Current Research Projects
• MRI: Acquisition of a flow field-flow
fractionation system to enhance colloidal research and education at USM. Funded by National
Science Foundation (NSF)-Major Research Instrument (MRI) program
(NSF-MRI #1126420) through OCE
Division of Ocean Sciences. $161,910 with additional 30% matching funds from the university, funding
period: January 2012-December 2013, (Principal Investigator with Co-PIs: Drs.
Orcutt and Shiller).
We propose to acquire an asymmetric flow
field-flow fractionation (FFF) system. This instrument will support
cross-disciplinary faculty research that integrates graduate and undergraduate
education with research. The requested instrument will add new research
capabilities, support and augment graduate education and ongoing research
projects in natural colloids and nanoparticles in aquatic systems. It has been
shown that colloids and nanoparticles are important intermediaries in
regulating the concentration and speciation and thus the fate, transport and
bioavailability/toxicity of many chemical species and contaminants in aquatic
environments. However, quantitative characterization of natural colloids and
knowledge of their environmental behavior remains scarce. Flow field-flow
fractionation is a chromatograph-like elution technique capable of simultaneous
separation and characterization of colloids, nanoparticles and macromolecules.
It has the advantages of providing a continuous size distribution or size
spectrum of colloids and other nanoparticles. By interfacing directly or
indirectly with other online or offline detection techniques such as ICP-MS and
gamma/alpha spectrometer, it will allow the elucidation of pathways and
mechanisms in the interactions between colloids/macromolecules and trace metals
and radionuclides in aquatic environments.
• Gulf of Mexico Research Initiatives (GoMRI/BP): Evaluating the changes in
fluorescence EEMs and size spectra of crude oil and dispersant during their
degradation in marine environments. March 2011 – April 2012, Principal investigator.
•
RAPID: Effect of oil
spill on organic carbon partitioning and transformation in the water column in
the northern Gulf. (NSF-OCE
#1042790), $60,000, funding period: 06/01/2010-05/31/2012 (no-cost
extension), principal investigator.
This is a Rapid Response Research
(RAPID) project funded by National Science Foundation
(NSF) Chemical
Oceanography Program (NSF-OCE#1042790).
We will examine the Deepwater Horizon oil spill in the northern Gulf of Mexico -- an oil spill of national significance
-- to study the fate, transport and transformation of crude oil components and
their interactions with marine organic matter from baseline to maximum impact
in a complete time series. Their working hypothesis is that the crude oil
components are isotopically light (in terms of carbon-13) and distinct in
optical properties (UV-vis and fluorescence) from natural marine organic matter,
thus allowing the use of stable isotopes and fluorescence techniques for
quantitative understanding of their fate, transport, and transformations in the
northern Gulf of Mexico. They will collect seawater samples at a station in the
Mississippi Bight in the northern Gulf of Mexico
from base line conditions to maximum impact. EEM fluorescence, stable isotopes,
and flow field-flow fractionation techniques will be employed. Results of this
study are expected to provide new insights into how organic matter from oil
spills interacts with marine organic matter affecting the ocean carbon budget,
biogeochemical processes, and marine ecosystems as a whole.
•
RAPID: Responses of Benthic
Communities and Sedimentary Dynamics to Hydrocarbon Exposure in Coastal Ecosystems
of the northern Gulf of Mexico. (NSF-OCE#1042907),
$147,874, June
2010-May 2011, (no-cost
extension), Co-PI with Drs.Yeager, Brunner, and Briggs.
This is a
RAPID award to respond to the Deep Horizon oil spill in the Gulf
of Mexico. The work involves the time series collection of samples
to examine the changes, from baseline conditions to maximum exposure, to the
oil leaking out of the deepwater well head to a variety of coastal sites along
the Northern Gulf of Mexico. Sampling sites
include the intertidal zone at Belle Fontaine Point in eastern Mississippi and the New
Harbor Islands
in Louisiana.
Samples and analytical work will determine hydrocarbon transformations and changes
due to biogeochemical processes, changes in bioturbation rates, and changes in
the assemblages of benthic macro- and meiofauna over time. Implications of
these variables for physical and sedimentary processes and for macrofauna will
be assessed. Broader impacts of the work will have immediate implications for
understanding the impacts of the oil spill on coastal ecosystems and provide
data to examine the time scale of ecosystem crash and recovery. The work will
have potential for mitigating the long term effects of the oil spill on coastal
systems. The work will also train students and provide public outreach
activities that will be carried out in collaboration with the Northern Gulf
Institution and with NOAA.
• 2010-2012:Time-series and Underway Assessments
of Ocean Acidification and Carbon System Properties in Coastal Waters
(supported by NOAA through Northern Gulf Institute). This project will provide time-series
observations of coastal ocean pH and carbon system properties in various
coastal regions in support of NOAA goals. While the majority of the work will
focus on the Gulf of Mexico, additional time-series sites will be maintained in
the South Atlantic Bight and Gulf
of Maine to provide a
comparison over a wide range of coastal and latitudinal regimes. 2010-2012
(Co-PI with Dr. Steve Lohrenz and Stephan Howden at USM).
•
Examining
the binding of radionuclides with marine biopolymers, A comparative study on
Th, Pa, Be, Po and Pb isotopes. (NSF-OCE#0850957), principal
investigator, funding period: 2009-2012.
Funded by National Science Foundation (NSF) Chemical
Oceanography Program (NSF-OCE#0850957).
Th(IV), Pa(IV,V), Po(IV, II,-II), Pb(II)
and Be(II) radioisotopes are important proxies in oceanographic investigations,
such as, for tracing particle dynamics and particulate organic matter (POC)
fluxes out of the euphotic zone, and for studying boundary scavenging,
paleo-productivity and ocean circulation. Even though considered routine, these
approaches rely on often poorly constrained, empirically determined and
variable isotope ratios or ratios to POC. Previously conducted laboratory and
field investigations suggest that a number of biopolymers, potentially produced
by both phytoplankton and bacteria, are carrier molecules for most of these
isotopes, rather than purely inorganic surfaces. Our hypothesis is that
specific binding and redox processes control marine scavenging and the most efficient
binding would occur to acid polysaccharide- and protein-containing
biomolecules. Proposed experiments will attempt to separate, identify and
characterize radioisotope carriers that are hypothesized to be effective
binding ligands. Our proposed interdisciplinary research project will require
instrumental approaches for characterization studies, in combination with
controlled laboratory and field experimentation. Laboratory studies consist of
comparative uptake experiments of a suite of naturally occurring radionuclides
to a number of substrates, including model organic and inorganic compounds,
marine colloidal and particulate organic matter, and biopolymers harvested from
cultures. The proposed field program will include collections and characterization
of diverse types of suspended and sinking organic matter from different parts
of the ocean.
• Coastal Impact Assistance Project (CIAP):
What is the state of health of the Bay
of St. Louis (2010-2014). This
project is supported by the Minerals Management Service/U.S. Department of
Interior, Co-PI with Dr.
Don Redalje and Dr. Stephan Howden
at USM
Recent Research Projects
• MRI-RAPID: Acquisition of Spectroscopic
Instrumentation for Oil Spill Research (NSF-MRI
#1057726), $108,871, funding period: 8/1/2010-7/31/2011, (Co-PI with Dr. Shiller).
Through
this NSF MRI-RAPID grant
and matching funds from University of Southern Mississippi (USM), we obtained several items of equipment to
be used to determine machine, water isotopes and colored dissolved organic
matter (CDOM) in oil spill samples in the Gulf of Mexico.
The requested instrumentation includes two cavity ring down spectrometers
(CRDS), one for determination of methane and the other for determination of the
oxygen and hydrogen isotopic composition of water. The CRDS methane analyzer is
needed because a significant fraction of material discharged from the Macondo
well is methane. The CRDS water isotope analyzer will allow the PI's to utilize
oxygen and hydrogen isotopes of water as a coastal water mass tracer. Both CRDS
instruments are robust enough to be used in the field. Using them in the field
will allow the PI's to adjust their sampling strategies based on the analytical
information obtained and to better understand water circulation/mixing and thus
oil transport and transformation in the northern Gulf of
Mexico. In addition, the PI's request a UV/visible
spectrophotometer. The spectrophotometer is a basic tool needed for determining
the optical properties of natural organic matter and oil-contaminated waters to
quantify the fate, transport and transformation of oil components.
• Monitoring
and Assessment of Coastal and Marine Ecosystems in the Northern Gulf”
(NGI/NOAA). This project is aimed at understanding the coastal nutrient,
carbon, and trace element fluxes in these several key environments, with an
emphasis on better understanding the transport and processing of nutrients and
pollutants through the coastal transition zone. A key element to this is the
role of fluvial inputs. Freshwater riverine input and it's associated dissolved
and particulate matter have a myriad of positive and negative effects on
coastal ecosystems. Basic steps in understanding the effects of inputs from
rivers and estuaries on coastal ecosystems include measuring the variability of
flow rates and concentrations of the constituents, tracking the concentrations
offshore from the input regions and determining the effects of freshwater input
to development of stratification and water quality variations. Time series of
these basic observations will be used to further understanding about the
processes transforming riverine input within the ecosystem of the northern Gulf of Mexico. More specifically, we will focus on
issues of coastal eutrophication, fluxes of carbon through the coastal
environment, and hypoxia in the Mississippi Bight. In collaboration with NOAA
scientist, Dr. Jia-Zhong Zhang, this sub-project supported by the Northern Gulf
Institute (NGI) during phase II (2009-2011) is part of the NGI project:
“Monitoring
and Assessment of Coastal and Marine Ecosystems in the Northern Gulf”,
Co-PI with Dr.
Stephan Howden and others at USM.
• Gulf of Mexico Research Initiative/BP:
Comprehensive
assessment of oil distribution, fate, transport and impacts on ecosystems.
This project was to i) characterize the
distribution and transport of the oil in coastal waters of the northern Gulf of
Mexico, ii) characterize the chemical form and evolution of oil and dispersant
and potential contribution to coastal hypoxia, iii) assess the impacts of oil
and dispersant on the coastal and offshore habitat, food webs, and living
marine resources, and iv) assess the impacts of the oil spill on public health
and welfare. Support from BP through the Northern Gulf Institute (NGI), June
2010-December 2011 (Lead PI, Steve Lohrenz). Click the link here
for more information.
• Spatial
variation and temporal trend of water quality in the northern Gulf of Mexico,
This is a research project supported by the National Oceanographic and
Atmospheric Administration (NOAA) through
the Northern
Gulf of Mexico (NGI), a NOAA Cooperative Institute. We proposed to conduct
ground truth observations and standardize algorithms to produce and evaluate
the spatial and temporal variations of water quality parameters (concentrations
of suspended organic (SOM) and inorganic matters (SIM), and water clarity) in
the Northern Gulf of Mexico, an effort aimed at improving the monitoring of
NGOM ecosystem based on remote sensing and understanding the dynamics of harmful
algae blooms in the northern Gulf of Mexico (Co-PI with Dr. Zhong-Ping Lee at Mississippi State University).
September 2009-September 2011.
• Composition and Fluxes of Organic
Carbon Species from the Yukon
River Basin (NSF-EAR#0403596
and EAR#0554781, 2004-2009)
Funded by National Science
Foundation (NSF) Integrated Carbon Cycle Research Program (2004-2009, Principal Investigator).
|

Sampling from the Yukon River, Pilot Station, Alaska

Yukon River Salmon
|
This project seeks to understand the biogeochemical
processes governing the composition, reactivity, transformation and flux of
organic carbon species, including dissolved, colloidal and particulate organic
carbon from the Yukon
River Basin. Being
heavily influenced by ice, snow and permafrost dynamics, the Yukon River
Basin, a pristine river basin, is sensitive to environmental and climate
change. With up to 50% of the world's soil organic carbon stored in the
northern region, the role of arctic/subarctic rivers in the remobilization of
organic carbon and thus marine and global carbon cycles is far more important
than previously recognized. Due to the Yukon River's remoteness and the
extreme weather conditions, the composition and reactivity of organic carbon
species entering the ocean via the Yukon
remain largely unknown and the riverine carbon export flux is poorly
quantified. Observations will include phase partitioning of
organic carbon between dissolved, colloidal and particulate phases, and
concentrations, optical properties, molecular and isotopic (d13C, d15N and
d14C) composition and reactivity of size fractionated organic carbon.
Field and laboratory experiments will also be carried out to examine the
extent to which organic carbon fractionates during ice formation and how the
fractionation drives carbon partitioning and transformation. This project
will be among the first to combine both molecular-level and basin-scale
observations to understand temporal and spatial variations in composition and
flux of organic carbon species in a large but pristine and less
anthropogenically influenced river basin. The proposed research will provide
valuable data for comparative studies between drainage basins, and provide
inputs for and contribute effectively to the integrated carbon cycle program
and model development, and the understanding of the environmental impacts
from a changing climate.
To learn more about global carbon cycle, click here. See
also IARC
research highlights.
|
|
Major publications from this project:
1. Belzile,
C. and Guo, L. 2006. Optical properties
of low molecular weight and colloidal organic matter: Application of the
ultrafiltration permeation model to DOM absorption and fluorescence. Marine Chemistry, 98, 183-196.
2. Gueguen,
C., Guo, L., Wang, D. Tanaka, N, and Hung, C.-C. 2006. Chemical
characteristics and origin of dissolved organic matter in the Yukon River. Biogeochemistry,
77, 139-155..
3.
Guo, L., and Macdonald, R.W. 2006. Source and
transport of terrigenous organic matter in the upper Yukon
River: evidence from isotope (13C, 14C and
15N) composition of dissolved, colloidal and particulate phases. Global Biogeochemical Cycles, 20,
GB2011, doi: 10.1029/2005GB002593.
4. Zou,
L., Sun, M.-Y. and Guo, L. 2006. Temporal variations of organic carbon
inputs into the upper Yukon River: Evidence
from fatty acids and their stable carbon isotopic composition in dissolved,
colloidal and particulate phases. Organic
Geochemistry, 37(8), 944-956.
5. Guo,
L. and Santschi, P.H. 2007. Ultrafiltration and its applications to sampling
and characterization of aquatic colloids. In "Environmental Colloids and
Particles," Wilkinson, K. and Lead, J.
(Eds), Chapter 4, p159-221, International Union of
Pure and Applied Chemistry (IUPAC) Series on
Analytical and Physical Chemistry of Environmental Systems, John
Wiley.
6.
Guo, L.D., Ping,
C.L., Macdonald, R.W. 2007. Mobilization pathways of organic carbon from
permafrost to arctic rivers in a changing climate. Geophys. Res. Lett., 34(13), L13603,
doi10.1029/2007GL030689.
7. Elmquist,
M., Semiletov, I., Guo, L.D. and Gustafsson, O. 2008. Pan-Arctic patterns in black carbon sources and fluvial
discharges deduced from radiocarbon and PAH source apportionment markers in
estuarine surface sediments. Global
Biogeochemical
Cycles, 22, GB2018, doi:10.1029/2007GB002994.
8.
Cai, Y., Guo, L.D. and Douglas,
T. 2008. Temporary variations in organic carbon species and fluxes from the
Chena River, Alaska. Limnology and
Oceanography, 53(4): 1408-1419.
9.
Cai, Y., Guo, L., Douglas,
T. and Whitledge, T. 2008. Seasonal variations in nutrient concentrations and
speciation in the Chena River, Alaska. JGR-Biogeosciences, 113, G030035, doi:10.1029/2008JG000733.
10. Xu,
C.H., Guo, L., Dou, F. and Ping,
C.-L. 2009. Potential DOC production from size fractionated Arctic tundra
soils, Alaska.
Cold Regions Science and Technology,
55, 141-150, doi:10.1016/j.coldregions.2008.08.001.
11. Xu,
C.H., Guo, L., Ping, C. L., and White, D. M. Chemical and isotopic characterization of size-fractionated organic matter
from cryoturbated soils, Northern Alaska. JGR-Biogeosciences, 114, G03002,
doi:10.1029/2008JG000846.
12. Cai, Y. and Guo, L. 2009. Abundance and
variation of colloidal organic phosphorus in riverine, estuarine and coastal
waters in the northern Gulf of Mexico. Limnology and Oceanography 54(4), 1393-1402.
13. McGuire, A.D., Anderson, L., Christensen, T.R., Dallimore,
S., Guo, L.D., Hayes, D., Heimann, M., Macdonald, R. and Roulet, N.
2009. Sensitivity of the Carbon Cycle in the Arctic
to Climate Change (Review). Ecological
Monographs, 79(4), 523-555.
14. Cooke,
M.P., van Dongen, B. E., Talbot, H.M., Semiletov, I.,
Guo, L. and Gustafsson, O. 2009. Bacteriohopanepolyol biomarker
composition of organic matter exported to the Arctic
Ocean by seven of the major Arctic rivers. Organic
Geochemistry, 40, 1151-1159,
doi:10.1016/j.orggeochem.2009.07.014,
(abstract or
pdf).
15. Guo, L., Cai, Y., Belzile, C. and Macdonald, R. 2010. Sources and export
fluxes of inorganic and organic carbon and nutrient species from the
seasonally ice-covered Yukon River. Biogeochemistry,,
doi:10.1007/s10533-010-9545-z
(abstract
or pdf
file).
|
• Collaborative Research: Th(IV) and Pa(IV, V)
binding to exopolymeric acid and polysaccharides in marine environments (NSF-OCE#0350758 or OCE#0627820,
2004-2009)
Funded by the National
Science Foundation (NSF) Chemical
Oceanography Program (2004-2009).
Th(IV) and Pa(IV,V) isotopes are important
proxies in oceanographic investigations, such as, for tracing particle dynamics
and particulate organic matter (POC) fluxes out of the euphotic zone through the
use of 234Th/POC ratios, and for studying boundary scavenging,
paleoproductivity and ocean circulation through the use of 231Pa/230Th ratios.
Even though almost routine, these approaches rely on often poorly constrained,
empirically determined and variable isotope ratios or ratios to POC. Previously
conducted laboratory and field investigations suggest that Th(IV) removal could
be controlled through binding by exopolymeric acid polysaccharide (APS) rich
biomolecules, potentially produced by both phytoplankton and bacteria. However,
we believe that Pa(V) present in ocean water must first be reduced to Pa(IV) by
organic biomolecules before efficient binding to solid phases can occur, and
that the most efficient binding would occur to APS-rich biomolecules produced
by phytoplankton species such as diatoms, prymnesiophytes and cyanobacteria. In
this study, the team of scientists at Texas A&M Research Foundation and the
University of Alaska Fairbanks Campus will investigate
the possible fractionation mechanisms between Pa(IV,V) and Th(IV) in the ocean.
It is essential to understand such a mechanism, since the Pa/Th ratio is
frequently used as a proxy in oceanographic applications. The proposed
interdisciplinary experimental approaches will require instrumental approaches
for characterization studies, in combination with controlled laboratory and
field experimentation. Laboratory studies consist of uptake experiments to a
number of substrates, including purified APS harvested from phytoplankton and
bacterial cultures to be used in Th(IV) and Pa(IV,V) binding assessments. The
most important analytical task will be to better characterize, both chemically,
in terms of molecular composition, and physically, in terms of surface
activity, the newly discovered strongly Th(IV) complexing APS of ~13 kDa
molecular weight, found in particulate and colloidal material collected from
the Gulf of Mexico, Atlantic and Pacific Ocean and the South China Sea. The
field program in this study will include collection and extraction of diverse
types of organic matter for use in laboratory studies, as well as the
determination of temporal and spatial variations of radiochemical and
biochemical parameters.
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Partitioning of Th-234 among dissolved, colloidal and particulate
phases in seawater
|

Fractionation between Th(IV) and
Pa(IV,V) scavenging in the ocean resulting from particulate composition
difference (from Guo et al. 2002)
|

Dr. Yihua Cai sampling during a 2006 cruise to the Gulf of Mexico on board R/V Seward Johnson (photo by L.
Guo)
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The R/V Seward
Johnson
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Publications from this NSF project
- Benitez-Nelson
C. and Th-234 Group. 2004. Future applications of Thorium-234 in aquatic
ecosystems. EOS, Transactions,
American Geophysical Union, 85 (45): 470-471.
- Hung,
C.C., Guo, L., Roberts, K.A. and Santschi, P.H. 2004. Upper ocean
carbon fluxes determined by the 234Th approach and sediment
traps using size fractionated POC and 234Th data from the Gulf of Mexico. Geochemical
Journal, 18(6): 601-611.
- Buesseler,
K. O., Benitez-Nelson, C., Moran, S. B., Burd, A. , Charette, M. ,
Cochran, J. K., Coppola, L., Fisher, N., Fowler, S. W., Gardner, W., Guo,
L. D., Gustafsson, O., Lamborg, C., Masque, P. , Miquel, J. C. ,
Passow, U., Santschi, P.H., Savoye, N., Stewart, G., and Trull, T..2006.
An assessment of particulate organic carbon to thorium-234 ratios in the
ocean and their impact on the application of 234Th as a POC
flux proxy. Marine Chemistry,
100, 213-233.
- Santschi,
P.H., Murray,
J.W., Baskaran, M.,Benitez-Nelson,
C., Guo, L.D., Hung, C.-C., Lamborg, C., Moran, B., Passow,
U., and Roy-Barman, M. 2006. Thorium speciation in seawater. Marine Chemistry, 100, 250-268.
- Wang,
D., Henrichs, S.M. and Guo, L. 2006. Distributions of nutrients,
dissolved organic carbon and carbohydrates in the western Arctic Ocean. Continental
Shelf Res., 26 (14), 1654-1667
- Guo,
L. and Santschi, P.H. 2007. Ultrafiltration and its applications to
sampling and characterization of aquatic colloids. In "Environmental
Colloids and Particles," Wilkinson,
K. and Lead, J. (Eds), Chapter 4, p159-221, International
Union of Pure and Applied Chemistry (IUPAC) Series on Analytical and Physical Chemistry of Environmental
Systems, John Wiley.
- Guo,
L.D., Warnken, K.W. and Santschi, P.H. 2007. Retention behavior of
dissolved uranium during ultrafiltration, implications for colloidal U in
surface waters. Marine Chemistry,
107(2), 156-166.
- Hung, C.-C., Moran, S. B., Cochran, J. K.,
Guo, L.D., and Santschi. P.H. 2008. Comment on: How accurate are 234Th
measurements in seawater based on the MnO2-impregnated
cartridge technique? by Pinghe Cai et al.” Geochemistry Geophysics Geosystems, 9, Q02009, doi10.1029/2007GC001770.
- Zou,
L., Guo, L.D., Sun, B.W. and Sun, M.Y. 2008, Origins of high-molecular-weight
dissolved organic matter in the Middle Atlantic Bight: Clue from lipids
and molecular d13C ratio. The Open
Oceanography Journal, 2, 1-5.
- Guo,
L. and M.-Y. Sun. 2009. Isotope composition of organic matter in
seawater. In: "Practical Guidelines
for the Analysis of Seawater", O. Wurl, editor, Chapter 6,
p97-123, CRC Press-Taylor and Francis Group, LLC.
- Guo,
L., White, D.M., Xu, C., and Santschi, P.H. 2009. Chemical and
isotopic composition of HMW-DOM from the Mississippi
River plume. Marine
Chemistry, 114, 63-71.
- Zhou, Z., Stolpe, B. and Guo, L. 2009.
Composition and size spectra of colored dissolved organic matter in river
waters as characterized by fluorescence EEM and flow field-flow
fractionation techniques. The 2009 Mississippi Water Conference,
August 5-7, 2009, Tunica, MS.
- Stolpe, B., Guo, L. Shiller, A. and Hassellov,
M. 2010. Size and composition of colloidal organic matter and Fe in the Mississippi and Pearl
Rivers and the northern Gulf of Mexico as characterized by flow-FFF techniques.
Marine Chemistry, 118:
119-228, doi: 10.1016/j.marchem.2009.11.007 (pdf online).
- Wang,
X., Cai, Y. and Guo, L.
2010. Preferential removal of dissolved carbohydrates during estuarine
mixing in the Bay of St Louis in the northern Gulf
of Mexico.. Marine
Chemistry, 119, 130-138,,
doi:10.1016/j.marchem.2010.01.006
(see Abstract
on line or pdf).
- Hung, C.-C.,
Xu, C., Santschi, P. H., Zhang, S., Schwehr, K.A., Quigg, A., Guo, L.,
Gong, G.-C., Pinckney, J., Long, R., and Wei, C.-L. 2010. Comparative evaluation of sediment-trap
and 234Th-derived POC fluxes from the upper oligotrophic waters
in the Gulf of Mexico and the subtropical northwestern Pacific
Ocean. Marine
Chemistry,121, 132-144,
doi:10.1016/j.marchem.2010.03.011.
• Flux and transformation of organic carbon across the
eroding coastline of northern Alaska
(NSF-OPP #0436179; 2004-2009).
Funded by the National Science Foundation (NSF) Arctic
System Science/Office of Polar Program
(2005-2009),
through collaboration with Drs. Chien-Lu
Ping and
Yuri Shur at UAF and Torre Jorgenson at ABR
Inc., Fairbanks (meet these
PIs). Our project is part of SNACS (Study of the
Northern Alaska Coastal System) Program, a contribution to the study of
environmental arctic change (SEARCH).
This proposed research addresses
scientific questions through four main components designed to: (1) characterize
the abundance, composition, and age of soil OC and the abundance and structure
of ground ice in relation to geomorphic environments, (2) estimate the total OC
flux along the entire Alaskan Beaufort Sea coast and develop empirical models
based on terrain and oceanographic factors to assess the vulnerability of the
coasts to increased erosion resulting from a longer fetch due to sea-ice
retreat, (3) to determine the biogeochemical transformation and bioavailability
of OC associated with various dissolved and particulate forms as they cross the
land/sea interface through field study and controlled laboratory
experimentation; and (4) integrate our results to the pan-arctic scale through
international collaboration with the Arctic Coastal Dynamics program. The study
will involve extensive sampling at 50 random locations along the entire coast
to develop precise estimates of OC abundance and flux with explicit confidence
limits. Intensive sampling at three key sites that represent the dominant
coastline types will be conducted to evaluate the transformation of the eroded
OC. Three additional secondary sites will be established to broaden the
monitoring to other coastline types and to involve local communities in the
assessment of coastal changes.
Broader Impacts: This project will provide information
critical to understanding of biogeochemical consequences of environmental
changes in the northern Alaska
that can be used to estimate pan-arctic coastal carbon and sediment inputs
through international collaboration with the Arctic Coastal Dynamics Program.
Results from this project will also increase our predictive capabilities in
related models that address the carbon cycle and the arctic climate system. Of
particular relevance will be the characterization of the bioavailability of
shallow- and deep-sequestered OC across a range of soil environments,
quantification of the variability of segregated and wedge ice that is essential
to assessing the terrain stability of northern Alaska subject to a warming
climate; and an improved understanding of the role of coastal erosion to the
input of carbon and nutrients to the Arctic Ocean. This project will integrate
research, professional training, and education through the participation of
graduate and undergraduate students in interdisciplinary and environmental
change research projects. An additional major part of this study will be
to develop a school and field curriculum for Barrow, Nuiqsut, and Kaktovik
residents, in partnership with the oil industry, local village liaisons, and
the U.S. Fish and Wildlife Service, to communicate study results and inform
local residents about the coastal processes that are import to marine
ecosystems and their subsistence activities.
Publications from this NSF project (Guo’s Group)
• Guo,
L.D., Ping, C.L., Macdonald, R.W. 2007.
Mobilization pathways of organic carbon from permafrost to arctic rivers in a
changing climate. Geophys. Res. Lett.,
34(13), L13603, doi10.1029/2007GL030689.
• Dou,
F., Ping, C.-L., Guo, L. and Jorgesson,
M.T. 2008. Estimating the impact of seawater on the
production of soil water-extractable organic carbon during coastal erosion.
Journal of Environmental Quality,
37(6), 2368-2374, doi:10.2134/jeq2007.0403.
• Elmquist, M., Semiletov, I., Guo, L.D. and
Gustafsson, O. 2008. Pan-Arctic
patterns in black carbon sources and fluvial discharges deduced from
radiocarbon and PAH source apportionment markers in estuarine surface
sediments.Global Biogeochemical Cycles,
22, GB2018, doi:10.1029/2007GB002994.
• Xu,
C.H., Guo, L., Dou, F. and Ping, C.-L.
2009. Potential DOC production from size fractionated Arctic tundra soils, Alaska. Cold Regions Science and Technology, 55, 141-150,
doi:10.1016/j.coldregions.2008.08.001.
• Xu,
C.H., Guo, L., Ping, C. L., and White,
D. M. 2009. Chemical and isotopic
characterization of size-fractionated organic matter from cryoturbated soils, Northern Alaska. JGR-Biogeosciences, 114, G03002, doi:10.1029/2008JG000846.
•
Cooke, M.P., van Dongen, B. E., Talbot, H.M., Semiletov, I.,
Guo, L. and Gustafsson, O.
2009. Bacteriohopanepolyol biomarker composition of organic matter exported to
the Arctic Ocean by seven of the major Arctic rivers.
Organic
Geochemistry, 40,
1151-1159,
doi:10.1016/j.orggeochem.2009.07.014,
(abstract or
pdf)
• Dou, F., Yu, X., Ping,
C.L., Michaelson, G., Guo, L. and Jorgenson, M. T. 2010. Spatial
variation of tundra soil organic carbon along the coastline of northern Alaska. Geoderma,
154: 328-335. doi: 10.1016/j.geoderma.2009.10.020.
• Zhang,
J.-Z., Guo, L. and Fischer,
C.R. 2010. Abundance and Chemical Speciation of Phosphorus in Sediments of the
Mackenzie Delta, Chukchi Sea and Bering Sea:
Importance of Detrital Apatite. Aquatic Geochemistry,
16: 353-371, doi: 10.1077/s10498-009-9081-4 (Abstract or
pdf online).
• Douglas, T.A., Fortier, D., Shur, Y.L.,
Kanevskiy, M.Z., Guo, L., Cai, Y. and Bray, M.T. 2011. Biogeochemical and geocryological
characteristics of wedge and thermokarst-cave ice in the CRREL Permafrost
Tunnel, Alaska. Permafrost
and Periglacial Processes, 21(4), doi:10.1002/ppp709 (abstract).
• Ping,
C.-L., G.J. Michaelson, L.D. Guo, M.T. Jorgenson, M. Kanevskiy, Y. Shur, F. Dou
and J. Liang. 2011. Soil carbon and material flux across the eroding coastline
of the Beaufort Sea, Alaska.
JGR-Biogeosciences,
doi:10.129/2010JG001588 (abstract or
pdf file).
|

Export of old terrigenous organic matter across the
arctic land/ocean interface: Evidence from estuarine sedimentary organic
carbon 14C ages along the Siberian Arctic coast (from Guo et al., 2004, Global Biogeochemical Cycles,
18(1), GB1036, pdf file).
|

Increasing coastal erotion under a changing climate in
the North
What are the impacts and
biogeochemical consequencies of climate and environmental change in the
north?
|
• Interactions of Th(IV) with organic
compound classes of marine organic matter (1999-2003)
A NSF Supported project (NSF-OCE #9906823): Th-234 has been widely used as a tracer to quantify the
fluxes of organic carbon export from the euphotic zone to the deep ocean in
many global research projects, such as Joint
Global Ocean Flux Study (JGOFS). Particulate organic carbon (POC)
fluxes are calculated as the product of the 234Th export flux times the
POC/234Th ratio. However, POC/234Th ratio in suspended and sinking particles
are not constant. It varies with different particle sizes, water depths, and
study areas. Understanding factors controlling the POC/234Th ratio in the ocean
is of paramount importance in global carbon cycle study and thus the climate
changes in the time scale of human concern.
The major objectives of the
proposed work are: 1) To collect colloidal, suspended and sinking particles
from marine environments for laboratory studies and to quantify the
relationships between the abundance of polysaccharide enriched marine
aggregates and Th(IV) deficiency (See photos of R/V Gyre 2000
and 2001Cruises); 2) To
conduct controlled laboratory experiments to investigate the interactions of Th
(IV) with individual organic compound classes of marine organic matter to
elucidate whether complexing of Th is a non-selective physicochemical process
(less likely) or organic ligand dependent (most likely); 3) To characterize the
bulk chemical, surface chemical, and isotopic composition of marine organic
matter to determine their acid-base and ligand properties and the nature of
organic or inorganic functional groups that Th (IV) tracks; 4) To construct an
improved scavenging model which relies on information on Th(IV) sorption to
strong surface active and non-surface active ligand groups in particulate and
colloidal organic matter. Experimental and model results will be used to
recommend improvements in the use of POC/234Thp ratios for POC flux
determinations.
• Role of natural organic matter in governing the bioavailability
of potentially toxic metals to estuarine bivalves (SeaGrant/NOAA, 2001-2004)
Supported by NOAA-Sea Grant: Bivalves have been extensively used as bio-indicator
organisms in environmental assessment and monitoring programs to assess the
bioavailable contaminant concentrations in coastal environments (e.g., NOAA's
NS&T program). Natural dissolved organic matter (DOM) is ubiquitous and is
a potentially nutritious food source for bivalves. However, the presence of DOM
may significantly alter the bioavailability and biogeochemical cycling pathways
of many trace metals in marine environments. The role of natural DOM in
governing the bioavailability of potentially toxic metals to bivalves is not
well understood and has rarely been tested. To better use bivalves as pollution
indicator organisms, a thorough understanding of metal uptake pathways and
mechanisms as a function of the quality and quantity of DOM is sorely needed.
The primary objectives of this research is to determine how DOM affects the
bioavailability of metals to bivalves, including oysters (Crasostrea
virginica) and mussels (Ischadium recurvum), and whether DOM can be
directly used as a food source by these bivalves using radiotracers and
molecular probes in controlled laboratory experiments. Our research will provide
crucial information applicable to environmental assessment and monitoring.
This is a research project supported by the NOAA-Sea Grant between
2001-2004, collaborating with Professor Peter Santschi
and Professor Sammy Ray in the
Laboratory for Oceanographic and Environmental Research (LOER) at the Texas A&M University at Galveston. My previous SeaGrant
supported project, namely "Bioavailability
of colloid-associated metals to estuarine bivalves" was completed during
1998-2001.
Ssee our publications in Environmental Science and
Technology (Guo et al. 2001, pdf)
and Marine
Environmental Research (Guo et al., 2002, pdf).
• Nature and fluxes of nutrients and organic matter from Yukon
River Basin (IARC, 2001-2004)
Principal investigator, Supported by Japan Frontier Research System for Global
Change/IARC: The Yukon River is one
of the largest rivers draining into the Arctic with an annual discharge of more
than 200 billion m3 of freshwater and ~60 million tons of suspended sediment,
and contributes ~8% of the total freshwater input to the Arctic Ocean.
Recent evidence shows that the polar region and the northern ecosystem are
quite sensitive to global and regional climate and environmental changes.
Therefore, environmental and climate changes in the northern arctic and thus increasing
freshwater flow and organic matter inputs from the Yukon River Basin will have
profound impacts on the ecosystem and biogeochemical cycles in the Bering Sea
and Arctic Ocean. However, due to its remoteness and the extreme weather
conditions, the Yukon
River Basin remains a
pristine and understudied basin, which can, in turn, serve as a natural
laboratory for arctic environmental change studies. We seek to understand
the nature and fluxes of nutrients (N, P, Si) and natural organic matter from
the Yukon River, as well as hydrological and water chemistry for a better
understanding of biogeochemical consequences of climate and resulting
environmental changes in the north. See our publications in Water
Researh (Guo et al., 2003, pdf),
Global
Biogeochemical Cycles (Guo et al, 2004, pdf) and Biogeochemistry
(Gueguen et al., 2005, pdf).
|

From Guo et al. (2004),
Speciation and fluxes of nutrients (N, P, Si) from the upper Yukon River, Global Biogeochemical Cycles,
18(1), GB1038 (pdf).
|

Winter sampling from Chena River, Alaska
|
• Radiocarbon and molecular characterization of organic compound
classes of dissolved organic matter in the ocean
Dissolved organic carbon (DOC) is the largest
organic C pool in the ocean and plays an important role in the global carbon
cycling. However, understanding the cycling of DOC in the ocean remains a major
challenge to oceanographers despite recent advances in marine organic carbon
cycling. To further explore the detailed biogeochemical pathways of different
organic components and to better understand the complex cycling of DOC in the
ocean, measurements of isotopic composition in different organic compound
classes are sorely needed, as are the chemical and molecular characterization
of different size or molecular weight fractionated DOC components.
The major objectives of the proposed research are: 1) To collect large
quantities of high molecular weight (HMW) dissolved organic matter from
seawater for chemical and isotopic characterizations, using cross-flow
ultrafiltration techniques; 2) To characterize elemental composition, major
organic compound classes and individual organic molecules using elemental
analysis, pyrolysis-GC/MS and liquid extraction techniques; 3) To determine
radiocarbon (14C) and stable carbon (d13C) and nitrogen (d15N) isotope
signatures of major organic compound classes, amino acids,
carbohydrates, total lipids, and acid-insoluble fractions in isolated HMW DOC
fractions; 4) To elucidate how chemical composition and isotope signatures of
the compound classes reflect their production and decomposition processes in
the ocean and to better understand the biogeochemical pathways and turnover
times of each compound class, whose geochemical behavior could be significantly
different from that of the total DOC pool.
This is a collaborative proposal declined by the National
Science Foundation (NSF) - Ocean
Science Division.
|

A schematic diagram of a ultrafiltration system for
isolating colloids and naoparticles from natural waters (from Guo 1995).
|

Freeze dried colloidal organic matter samples
from estuarine seawater
|

Atomic force microscopy image of aquatic colloids
(from Santschi et al., 1998, L&O)
|
Research collaborators
Major Equipment
Total organic carbon and nitrogen
analyzer (Shimadzu TOC-V) with
capability of measuring DOC and TN;
Canberra
gamma counting system, with ultrahigh purity Ge well detector and multichannel
analyzer;
Shimadzu RF-5301 Spectroflurometer;
Aglient 8453
Spectrophotometer;
TIDAS-LWCC High Performance fiber
optic spectrophotometer (World Precision Instruments)
Autoanalyzer (Seal) for nutrient analysis;
Flow field-flow fractionation
(FlFFF) and SPLITT systems (Postnova
Analytics) which can be
coupled with on-line detectors such as UV absorbance, fluoresce, refractive
index, light scattering. and ICP-MS, providing continuum size spectra in
colloidal and nanoparticle materials in natural waters.
Cross-flow ultrafiltration
systems (e.g., Millipore Proflux
M-30 and Amicon DC-10 systems);
Ultra-pure water system,
ultracentrifuge, freeze dryer, clean benches, auto balances, etc.
See also Center for Trace Analysis at USM
|

CTD deployment during a research cruise to the Arctic Ocean aboard R/V Mirai (JAMSTEC).
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Ultrafiltration system for processing large
volume of seawater to isolate sufficient quantities of colloidal organic
matter
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This page Copyright © 2001-2011 Laodong
Guo.
Last updated Wednesday, June 01, 2011