"Organic soup" from the Yukon River

Dr. Laodong Guo

Professor

School of Freshwater Sciences (SFS)
University of Wisconsin-Milwaukee (UWM)
600 East Greenfield Avenue
Milwaukee, WI 53204, USA

Tel:     (414)-382-1700
Fax:    (414)-382-1705
E-mail: guol@uwm.edu



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Current Research Projects

MRI: Acquisition of a flow field-flow fractionation system to enhance colloidal research and education at USM. Funded by National Science Foundation (NSF)-Major Research Instrument (MRI) program (NSF-MRI #1126420) through OCE Division of Ocean Sciences. $161,910 with additional 30% matching funds from the university, funding period: January 2012-December 2013, (Principal Investigator with Co-PIs: Drs. Orcutt and Shiller).

We propose to acquire an asymmetric flow field-flow fractionation (FFF) system. This instrument will support cross-disciplinary faculty research that integrates graduate and undergraduate education with research. The requested instrument will add new research capabilities, support and augment graduate education and ongoing research projects in natural colloids and nanoparticles in aquatic systems. It has been shown that colloids and nanoparticles are important intermediaries in regulating the concentration and speciation and thus the fate, transport and bioavailability/toxicity of many chemical species and contaminants in aquatic environments. However, quantitative characterization of natural colloids and knowledge of their environmental behavior remains scarce. Flow field-flow fractionation is a chromatograph-like elution technique capable of simultaneous separation and characterization of colloids, nanoparticles and macromolecules. It has the advantages of providing a continuous size distribution or size spectrum of colloids and other nanoparticles. By interfacing directly or indirectly with other online or offline detection techniques such as ICP-MS and gamma/alpha spectrometer, it will allow the elucidation of pathways and mechanisms in the interactions between colloids/macromolecules and trace metals and radionuclides in aquatic environments.

Gulf of Mexico Research Initiatives (GoMRI/BP): Evaluating the changes in fluorescence EEMs and size spectra of crude oil and dispersant during their degradation in marine environments. March 2011 – April 2012, Principal investigator.

RAPID: Effect of oil spill on organic carbon partitioning and transformation in the water column in the northern Gulf. (NSF-OCE #1042790), $60,000, funding period: 06/01/2010-05/31/2012 (no-cost extension), principal investigator.

This is a Rapid Response Research (RAPID) project funded by National Science Foundation (NSF) Chemical Oceanography Program (NSF-OCE#1042790). We will examine the Deepwater Horizon oil spill in the northern Gulf of Mexico -- an oil spill of national significance -- to study the fate, transport and transformation of crude oil components and their interactions with marine organic matter from baseline to maximum impact in a complete time series. Their working hypothesis is that the crude oil components are isotopically light (in terms of carbon-13) and distinct in optical properties (UV-vis and fluorescence) from natural marine organic matter, thus allowing the use of stable isotopes and fluorescence techniques for quantitative understanding of their fate, transport, and transformations in the northern Gulf of Mexico. They will collect seawater samples at a station in the Mississippi Bight in the northern Gulf of Mexico from base line conditions to maximum impact. EEM fluorescence, stable isotopes, and flow field-flow fractionation techniques will be employed. Results of this study are expected to provide new insights into how organic matter from oil spills interacts with marine organic matter affecting the ocean carbon budget, biogeochemical processes, and marine ecosystems as a whole.

RAPID: Responses of Benthic Communities and Sedimentary Dynamics to Hydrocarbon Exposure in Coastal Ecosystems of the northern Gulf of Mexico. (NSF-OCE#1042907), $147,874, June 2010-May 2011, (no-cost extension), Co-PI with Drs.Yeager, Brunner, and Briggs.

This is a RAPID award to respond to the Deep Horizon oil spill in the Gulf of Mexico. The work involves the time series collection of samples to examine the changes, from baseline conditions to maximum exposure, to the oil leaking out of the deepwater well head to a variety of coastal sites along the Northern Gulf of Mexico. Sampling sites include the intertidal zone at Belle Fontaine Point in eastern Mississippi and the New Harbor Islands in Louisiana. Samples and analytical work will determine hydrocarbon transformations and changes due to biogeochemical processes, changes in bioturbation rates, and changes in the assemblages of benthic macro- and meiofauna over time. Implications of these variables for physical and sedimentary processes and for macrofauna will be assessed. Broader impacts of the work will have immediate implications for understanding the impacts of the oil spill on coastal ecosystems and provide data to examine the time scale of ecosystem crash and recovery. The work will have potential for mitigating the long term effects of the oil spill on coastal systems. The work will also train students and provide public outreach activities that will be carried out in collaboration with the Northern Gulf Institution and with NOAA.

 

• 2010-2012:Time-series and Underway Assessments of Ocean Acidification and Carbon System Properties in Coastal Waters (supported by NOAA through Northern Gulf Institute). This project will provide time-series observations of coastal ocean pH and carbon system properties in various coastal regions in support of NOAA goals. While the majority of the work will focus on the Gulf of Mexico, additional time-series sites will be maintained in the South Atlantic Bight and Gulf of Maine to provide a comparison over a wide range of coastal and latitudinal regimes. 2010-2012 (Co-PI with Dr. Steve Lohrenz and Stephan Howden at USM).

 

Examining the binding of radionuclides with marine biopolymers, A comparative study on Th, Pa, Be, Po and Pb isotopes. (NSF-OCE#0850957), principal investigator, funding period: 2009-2012.

Funded by National Science Foundation (NSF) Chemical Oceanography Program (NSF-OCE#0850957). Th(IV), Pa(IV,V), Po(IV, II,-II), Pb(II) and Be(II) radioisotopes are important proxies in oceanographic investigations, such as, for tracing particle dynamics and particulate organic matter (POC) fluxes out of the euphotic zone, and for studying boundary scavenging, paleo-productivity and ocean circulation. Even though considered routine, these approaches rely on often poorly constrained, empirically determined and variable isotope ratios or ratios to POC. Previously conducted laboratory and field investigations suggest that a number of biopolymers, potentially produced by both phytoplankton and bacteria, are carrier molecules for most of these isotopes, rather than purely inorganic surfaces. Our hypothesis is that specific binding and redox processes control marine scavenging and the most efficient binding would occur to acid polysaccharide- and protein-containing biomolecules. Proposed experiments will attempt to separate, identify and characterize radioisotope carriers that are hypothesized to be effective binding ligands. Our proposed interdisciplinary research project will require instrumental approaches for characterization studies, in combination with controlled laboratory and field experimentation. Laboratory studies consist of comparative uptake experiments of a suite of naturally occurring radionuclides to a number of substrates, including model organic and inorganic compounds, marine colloidal and particulate organic matter, and biopolymers harvested from cultures. The proposed field program will include collections and characterization of diverse types of suspended and sinking organic matter from different parts of the ocean.

 

Coastal Impact Assistance Project (CIAP): What is the state of health of the Bay of St. Louis (2010-2014). This project is supported by the Minerals Management Service/U.S. Department of Interior, Co-PI with Dr. Don Redalje and Dr. Stephan Howden at USM

 

 

Recent Research Projects

MRI-RAPID: Acquisition of Spectroscopic Instrumentation for Oil Spill Research (NSF-MRI #1057726), $108,871, funding period: 8/1/2010-7/31/2011, (Co-PI with Dr. Shiller).

Through this NSF MRI-RAPID grant and matching funds from University of Southern Mississippi (USM), we obtained several items of equipment to be used to determine machine, water isotopes and colored dissolved organic matter (CDOM) in oil spill samples in the Gulf of Mexico. The requested instrumentation includes two cavity ring down spectrometers (CRDS), one for determination of methane and the other for determination of the oxygen and hydrogen isotopic composition of water. The CRDS methane analyzer is needed because a significant fraction of material discharged from the Macondo well is methane. The CRDS water isotope analyzer will allow the PI's to utilize oxygen and hydrogen isotopes of water as a coastal water mass tracer. Both CRDS instruments are robust enough to be used in the field. Using them in the field will allow the PI's to adjust their sampling strategies based on the analytical information obtained and to better understand water circulation/mixing and thus oil transport and transformation in the northern Gulf of Mexico. In addition, the PI's request a UV/visible spectrophotometer. The spectrophotometer is a basic tool needed for determining the optical properties of natural organic matter and oil-contaminated waters to quantify the fate, transport and transformation of oil components.

 

 

Monitoring and Assessment of Coastal and Marine Ecosystems in the Northern Gulf (NGI/NOAA). This project is aimed at understanding the coastal nutrient, carbon, and trace element fluxes in these several key environments, with an emphasis on better understanding the transport and processing of nutrients and pollutants through the coastal transition zone. A key element to this is the role of fluvial inputs. Freshwater riverine input and it's associated dissolved and particulate matter have a myriad of positive and negative effects on coastal ecosystems. Basic steps in understanding the effects of inputs from rivers and estuaries on coastal ecosystems include measuring the variability of flow rates and concentrations of the constituents, tracking the concentrations offshore from the input regions and determining the effects of freshwater input to development of stratification and water quality variations. Time series of these basic observations will be used to further understanding about the processes transforming riverine input within the ecosystem of the northern Gulf of Mexico. More specifically, we will focus on issues of coastal eutrophication, fluxes of carbon through the coastal environment, and hypoxia in the Mississippi Bight. In collaboration with NOAA scientist, Dr. Jia-Zhong Zhang, this sub-project supported by the Northern Gulf Institute (NGI) during phase II (2009-2011) is part of the NGI project: “Monitoring and Assessment of Coastal and Marine Ecosystems in the Northern Gulf”, Co-PI with Dr. Stephan Howden and others at USM.

 

 

Gulf of Mexico Research Initiative/BP: Comprehensive assessment of oil distribution, fate, transport and impacts on ecosystems. This project was to  i) characterize the distribution and transport of the oil in coastal waters of the northern Gulf of Mexico, ii) characterize the chemical form and evolution of oil and dispersant and potential contribution to coastal hypoxia, iii) assess the impacts of oil and dispersant on the coastal and offshore habitat, food webs, and living marine resources, and iv) assess the impacts of the oil spill on public health and welfare. Support from BP through the Northern Gulf Institute (NGI), June 2010-December 2011 (Lead PI, Steve Lohrenz). Click the link here for more information.

 

Spatial variation and temporal trend of water quality in the northern Gulf of Mexico, This is a research project supported by the National Oceanographic and Atmospheric Administration (NOAA) through the Northern Gulf of Mexico (NGI), a NOAA Cooperative Institute. We proposed to conduct ground truth observations and standardize algorithms to produce and evaluate the spatial and temporal variations of water quality parameters (concentrations of suspended organic (SOM) and inorganic matters (SIM), and water clarity) in the Northern Gulf of Mexico, an effort aimed at improving the monitoring of NGOM ecosystem based on remote sensing and understanding the dynamics of harmful algae blooms in the northern Gulf of Mexico (Co-PI with Dr. Zhong-Ping Lee at Mississippi State University). September 2009-September 2011.

 

Composition and Fluxes of Organic Carbon Species from the Yukon River Basin (NSF-EAR#0403596 and EAR#0554781, 2004-2009)

Funded by National Science Foundation (NSF) Integrated Carbon Cycle Research Program (2004-2009, Principal Investigator).


Sampling from the Yukon River, Pilot Station, Alaska


Yukon River Salmon

 

This project seeks to understand the biogeochemical processes governing the composition, reactivity, transformation and flux of organic carbon species, including dissolved, colloidal and particulate organic carbon from the Yukon River Basin. Being heavily influenced by ice, snow and permafrost dynamics, the Yukon River Basin, a pristine river basin, is sensitive to environmental and climate change. With up to 50% of the world's soil organic carbon stored in the northern region, the role of arctic/subarctic rivers in the remobilization of organic carbon and thus marine and global carbon cycles is far more important than previously recognized. Due to the Yukon River's remoteness and the extreme weather conditions, the composition and reactivity of organic carbon species entering the ocean via the Yukon remain largely unknown and the riverine carbon export flux is poorly quantified.   Observations will include phase partitioning of organic carbon between dissolved, colloidal and particulate phases, and concentrations, optical properties, molecular and isotopic (d13C, d15N and d14C) composition and reactivity of size fractionated organic carbon.  Field and laboratory experiments will also be carried out to examine the extent to which organic carbon fractionates during ice formation and how the fractionation drives carbon partitioning and transformation. This project will be among the first to combine both molecular-level and basin-scale observations to understand temporal and spatial variations in composition and flux of organic carbon species in a large but pristine and less anthropogenically influenced river basin. The proposed research will provide valuable data for comparative studies between drainage basins, and provide inputs for and contribute effectively to the integrated carbon cycle program and model development, and the understanding of the environmental impacts from a changing climate.

 

To learn more about global carbon cycle, click here.  See also  IARC research highlights.

 

 

Major publications from this project:

1.      Belzile, C. and Guo, L. 2006. Optical properties of low molecular weight and colloidal organic matter: Application of the ultrafiltration permeation model to DOM absorption and fluorescence. Marine Chemistry, 98, 183-196.

2.      Gueguen, C., Guo, L., Wang, D. Tanaka, N, and Hung, C.-C. 2006. Chemical characteristics and origin of dissolved organic matter in the Yukon River. Biogeochemistry, 77, 139-155..

3.      Guo, L., and Macdonald, R.W. 2006. Source and transport of terrigenous organic matter in the upper Yukon River: evidence from isotope (13C, 14C and 15N) composition of dissolved, colloidal and particulate phases. Global Biogeochemical Cycles, 20, GB2011, doi: 10.1029/2005GB002593.

4.      Zou, L., Sun, M.-Y. and Guo, L. 2006. Temporal variations of organic carbon inputs into the upper Yukon River: Evidence from fatty acids and their stable carbon isotopic composition in dissolved, colloidal and particulate phases. Organic Geochemistry, 37(8), 944-956.

5.      Guo, L. and Santschi, P.H. 2007. Ultrafiltration and its applications to sampling and characterization of aquatic colloids. In "Environmental Colloids and Particles," Wilkinson, K. and Lead, J. (Eds), Chapter 4, p159-221, International Union of Pure and Applied Chemistry (IUPAC) Series on Analytical and Physical Chemistry of Environmental Systems, John Wiley.

6.      Guo, L.D., Ping, C.L., Macdonald, R.W. 2007. Mobilization pathways of organic carbon from permafrost to arctic rivers in a changing climate. Geophys. Res. Lett., 34(13), L13603, doi10.1029/2007GL030689.

7.      Elmquist, M., Semiletov, I., Guo, L.D. and Gustafsson, O. 2008. Pan-Arctic patterns in black carbon sources and fluvial discharges deduced from radiocarbon and PAH source apportionment markers in estuarine surface sediments. Global Biogeochemical Cycles, 22, GB2018, doi:10.1029/2007GB002994.

8.      Cai, Y., Guo, L.D. and Douglas, T. 2008. Temporary variations in organic carbon species and fluxes from the Chena River, Alaska. Limnology and Oceanography, 53(4): 1408-1419.

9.      Cai, Y., Guo, L., Douglas, T. and Whitledge, T. 2008. Seasonal variations in nutrient concentrations and speciation in the Chena River, Alaska. JGR-Biogeosciences, 113, G030035, doi:10.1029/2008JG000733.

10.  Xu, C.H., Guo, L., Dou, F. and Ping, C.-L. 2009. Potential DOC production from size fractionated Arctic tundra soils, Alaska. Cold Regions Science and Technology, 55, 141-150, doi:10.1016/j.coldregions.2008.08.001.

11.  Xu, C.H., Guo, L., Ping, C. L., and White, D. M. Chemical and isotopic characterization of size-fractionated organic matter from cryoturbated soils, Northern Alaska. JGR-Biogeosciences, 114, G03002, doi:10.1029/2008JG000846.

12.  Cai, Y. and Guo, L. 2009. Abundance and variation of colloidal organic phosphorus in riverine, estuarine and coastal waters in the northern Gulf of Mexico. Limnology and Oceanography 54(4), 1393-1402.

13.  McGuire, A.D., Anderson, L., Christensen, T.R., Dallimore, S., Guo, L.D., Hayes, D., Heimann, M., Macdonald, R. and Roulet, N. 2009. Sensitivity of the Carbon Cycle in the Arctic to Climate Change (Review). Ecological Monographs, 79(4), 523-555.

14.  Cooke, M.P., van Dongen, B. E., Talbot, H.M., Semiletov, I., Guo, L. and Gustafsson, O. 2009. Bacteriohopanepolyol biomarker composition of organic matter exported to the Arctic Ocean by seven of the major Arctic rivers. Organic Geochemistry, 40, 1151-1159, doi:10.1016/j.orggeochem.2009.07.014, (abstract or pdf).

15.  Guo, L., Cai, Y., Belzile, C. and Macdonald, R. 2010. Sources and export fluxes of inorganic and organic carbon and nutrient species from the seasonally ice-covered Yukon River. Biogeochemistry,, doi:10.1007/s10533-010-9545-z (abstract or pdf file).

Collaborative Research: Th(IV) and Pa(IV, V) binding to exopolymeric acid and polysaccharides in marine environments  (NSF-OCE#0350758 or OCE#0627820, 2004-2009)

Funded by the National Science Foundation (NSF) Chemical Oceanography Program (2004-2009).
Th(IV) and Pa(IV,V) isotopes are important proxies in oceanographic investigations, such as, for tracing particle dynamics and particulate organic matter (POC) fluxes out of the euphotic zone through the use of 234Th/POC ratios, and for studying boundary scavenging, paleoproductivity and ocean circulation through the use of 231Pa/230Th ratios. Even though almost routine, these approaches rely on often poorly constrained, empirically determined and variable isotope ratios or ratios to POC. Previously conducted laboratory and field investigations suggest that Th(IV) removal could be controlled through binding by exopolymeric acid polysaccharide (APS) rich biomolecules, potentially produced by both phytoplankton and bacteria. However, we believe that Pa(V) present in ocean water must first be reduced to Pa(IV) by organic biomolecules before efficient binding to solid phases can occur, and that the most efficient binding would occur to APS-rich biomolecules produced by phytoplankton species such as diatoms, prymnesiophytes and cyanobacteria. In this study, the team of scientists at Texas A&M Research Foundation and the University of Alaska Fairbanks Campus will investigate the possible fractionation mechanisms between Pa(IV,V) and Th(IV) in the ocean. It is essential to understand such a mechanism, since the Pa/Th ratio is frequently used as a proxy in oceanographic applications. The proposed interdisciplinary experimental approaches will require instrumental approaches for characterization studies, in combination with controlled laboratory and field experimentation. Laboratory studies consist of uptake experiments to a number of substrates, including purified APS harvested from phytoplankton and bacterial cultures to be used in Th(IV) and Pa(IV,V) binding assessments. The most important analytical task will be to better characterize, both chemically, in terms of molecular composition, and physically, in terms of surface activity, the newly discovered strongly Th(IV) complexing APS of ~13 kDa molecular weight, found in particulate and colloidal material collected from the Gulf of Mexico, Atlantic and Pacific Ocean and the South China Sea. The field program in this study will include collection and extraction of diverse types of organic matter for use in laboratory studies, as well as the determination of temporal and spatial variations of radiochemical and biochemical parameters.

 

Partitioning of Th-234 among dissolved, colloidal and particulate phases in seawater

Fractionation between Th(IV) and Pa(IV,V) scavenging in the ocean resulting from particulate composition difference (from Guo et al. 2002)

Dr. Yihua Cai sampling during a 2006 cruise to the Gulf of Mexico on board R/V Seward Johnson (photo by L. Guo)

The R/V Seward Johnson

 

Publications from this NSF project

  1. Benitez-Nelson C. and Th-234 Group. 2004. Future applications of Thorium-234 in aquatic ecosystems. EOS, Transactions, American Geophysical Union, 85 (45): 470-471.
  2. Hung, C.C., Guo, L., Roberts, K.A. and Santschi, P.H. 2004. Upper ocean carbon fluxes determined by the 234Th approach and sediment traps using size fractionated POC and 234Th data from the Gulf of Mexico. Geochemical Journal, 18(6): 601-611.
  3. Buesseler, K. O., Benitez-Nelson, C., Moran, S. B., Burd, A. , Charette, M. , Cochran, J. K., Coppola, L., Fisher, N., Fowler, S. W., Gardner, W., Guo, L. D., Gustafsson, O., Lamborg, C., Masque, P. , Miquel, J. C. , Passow, U., Santschi, P.H., Savoye, N., Stewart, G., and Trull, T..2006. An assessment of particulate organic carbon to thorium-234 ratios in the ocean and their impact on the application of 234Th as a POC flux proxy. Marine Chemistry, 100, 213-233.
  4. Santschi, P.H., Murray, J.W., Baskaran, M.,Benitez-Nelson, C., Guo, L.D., Hung, C.-C., Lamborg, C., Moran, B., Passow, U., and Roy-Barman, M. 2006. Thorium speciation in seawater. Marine Chemistry, 100, 250-268.
  5. Wang, D., Henrichs, S.M. and Guo, L. 2006. Distributions of nutrients, dissolved organic carbon and carbohydrates in the western Arctic Ocean. Continental Shelf Res., 26 (14), 1654-1667
  6. Guo, L. and Santschi, P.H. 2007. Ultrafiltration and its applications to sampling and characterization of aquatic colloids. In "Environmental Colloids and Particles," Wilkinson, K. and Lead, J. (Eds), Chapter 4, p159-221, International Union of Pure and Applied Chemistry (IUPAC) Series on Analytical and Physical Chemistry of Environmental Systems, John Wiley.
  7. Guo, L.D., Warnken, K.W. and Santschi, P.H. 2007. Retention behavior of dissolved uranium during ultrafiltration, implications for colloidal U in surface waters. Marine Chemistry, 107(2), 156-166.
  8. Hung, C.-C., Moran, S. B., Cochran, J. K., Guo, L.D., and Santschi. P.H. 2008. Comment on: How accurate are 234Th measurements in seawater based on the MnO2-impregnated cartridge technique? by Pinghe Cai et al.” Geochemistry Geophysics Geosystems, 9, Q02009, doi10.1029/2007GC001770.
  9. Zou, L., Guo, L.D., Sun, B.W. and Sun, M.Y. 2008, Origins of high-molecular-weight dissolved organic matter in the Middle Atlantic Bight: Clue from lipids and molecular d13C ratio. The Open Oceanography Journal, 2, 1-5.
  10. Guo, L. and M.-Y. Sun. 2009. Isotope composition of organic matter in seawater. In: "Practical Guidelines for the Analysis of Seawater", O. Wurl, editor, Chapter 6, p97-123, CRC Press-Taylor and Francis Group, LLC.
  11. Guo, L., White, D.M., Xu, C., and Santschi, P.H. 2009. Chemical and isotopic composition of HMW-DOM from the Mississippi River plume. Marine Chemistry, 114, 63-71.
  12. Zhou, Z., Stolpe, B. and Guo, L. 2009. Composition and size spectra of colored dissolved organic matter in river waters as characterized by fluorescence EEM and flow field-flow fractionation techniques. The 2009 Mississippi Water Conference, August 5-7, 2009, Tunica, MS.
  13. Stolpe, B., Guo, L. Shiller, A. and Hassellov, M. 2010. Size and composition of colloidal organic matter and Fe in the Mississippi and Pearl Rivers and the northern Gulf of Mexico as characterized by flow-FFF techniques. Marine Chemistry, 118: 119-228, doi: 10.1016/j.marchem.2009.11.007 (pdf online).
  14. Wang, X., Cai, Y. and Guo, L. 2010. Preferential removal of dissolved carbohydrates during estuarine mixing in the Bay of St Louis in the northern Gulf of Mexico.. Marine Chemistry, 119, 130-138,, doi:10.1016/j.marchem.2010.01.006 (see Abstract on line or pdf).
  15. Hung, C.-C., Xu, C., Santschi, P. H., Zhang, S., Schwehr, K.A., Quigg, A., Guo, L., Gong, G.-C., Pinckney, J., Long, R., and Wei, C.-L. 2010. Comparative evaluation of sediment-trap and 234Th-derived POC fluxes from the upper oligotrophic waters in the Gulf of Mexico and the subtropical northwestern Pacific Ocean. Marine Chemistry,121, 132-144, doi:10.1016/j.marchem.2010.03.011.



Flux and transformation of organic carbon across the eroding coastline of northern Alaska (NSF-OPP #0436179; 2004-2009).


Funded by the
National Science Foundation (NSF) Arctic System Science/Office of Polar Program (2005-2009), through collaboration with Drs. Chien-Lu Ping and Yuri Shur at UAF and Torre Jorgenson at ABR Inc., Fairbanks (meet these PIs).  Our project is part of SNACS (Study of the Northern Alaska Coastal System) Program, a contribution to the study of environmental arctic change (SEARCH).

This proposed research addresses scientific questions through four main components designed to: (1) characterize the abundance, composition, and age of soil OC and the abundance and structure of ground ice in relation to geomorphic environments, (2) estimate the total OC flux along the entire Alaskan Beaufort Sea coast and develop empirical models based on terrain and oceanographic factors to assess the vulnerability of the coasts to increased erosion resulting from a longer fetch due to sea-ice retreat, (3) to determine the biogeochemical transformation and bioavailability of OC associated with various dissolved and particulate forms as they cross the land/sea interface through field study and controlled laboratory experimentation; and (4) integrate our results to the pan-arctic scale through international collaboration with the Arctic Coastal Dynamics program. The study will involve extensive sampling at 50 random locations along the entire coast to develop precise estimates of OC abundance and flux with explicit confidence limits. Intensive sampling at three key sites that represent the dominant coastline types will be conducted to evaluate the transformation of the eroded OC.  Three additional secondary sites will be established to broaden the monitoring to other coastline types and to involve local communities in the assessment of coastal changes.
Broader Impacts:  This project will provide information critical to understanding of biogeochemical consequences of environmental changes in the northern Alaska that can be used to estimate pan-arctic coastal carbon and sediment inputs through international collaboration with the Arctic Coastal Dynamics Program. Results from this project will also increase our predictive capabilities in related models that address the carbon cycle and the arctic climate system. Of particular relevance will be the characterization of the bioavailability of shallow- and deep-sequestered OC across a range of soil environments, quantification of the variability of segregated and wedge ice that is essential to assessing the terrain stability of northern Alaska subject to a warming climate; and an improved understanding of the role of coastal erosion to the input of carbon and nutrients to the Arctic Ocean. This project will integrate research, professional training, and education through the participation of graduate and undergraduate students in interdisciplinary and environmental change research projects.  An additional major part of this study will be to develop a school and field curriculum for Barrow, Nuiqsut, and Kaktovik residents, in partnership with the oil industry, local village liaisons, and the U.S. Fish and Wildlife Service, to communicate study results and inform local residents about the coastal processes that are import to marine ecosystems and their subsistence activities.

Publications from this NSF project (Guo’s Group)

Guo, L.D., Ping, C.L., Macdonald, R.W. 2007. Mobilization pathways of organic carbon from permafrost to arctic rivers in a changing climate. Geophys. Res. Lett., 34(13), L13603, doi10.1029/2007GL030689.

• Dou, F., Ping, C.-L., Guo, L. and Jorgesson, M.T. 2008. Estimating the impact of seawater on the production of soil water-extractable organic carbon during coastal erosion. Journal of Environmental Quality, 37(6), 2368-2374, doi:10.2134/jeq2007.0403.

Elmquist, M., Semiletov, I., Guo, L.D. and Gustafsson, O. 2008. Pan-Arctic patterns in black carbon sources and fluvial discharges deduced from radiocarbon and PAH source apportionment markers in estuarine surface sediments.Global Biogeochemical Cycles, 22, GB2018, doi:10.1029/2007GB002994.

• Xu, C.H., Guo, L., Dou, F. and Ping, C.-L. 2009. Potential DOC production from size fractionated Arctic tundra soils, Alaska. Cold Regions Science and Technology, 55, 141-150, doi:10.1016/j.coldregions.2008.08.001.

• Xu, C.H., Guo, L., Ping, C. L., and White, D. M. 2009. Chemical and isotopic characterization of size-fractionated organic matter from cryoturbated soils, Northern Alaska. JGR-Biogeosciences, 114, G03002, doi:10.1029/2008JG000846.

• Cooke, M.P., van Dongen, B. E., Talbot, H.M., Semiletov, I., Guo, L. and Gustafsson, O. 2009. Bacteriohopanepolyol biomarker composition of organic matter exported to the Arctic Ocean by seven of the major Arctic rivers. Organic Geochemistry, 40, 1151-1159, doi:10.1016/j.orggeochem.2009.07.014, (abstract or pdf)

Dou, F., Yu, X., Ping, C.L., Michaelson, G., Guo, L. and Jorgenson, M. T. 2010. Spatial variation of tundra soil organic carbon along the coastline of northern Alaska. Geoderma, 154: 328-335. doi: 10.1016/j.geoderma.2009.10.020.

• Zhang, J.-Z., Guo, L. and Fischer, C.R. 2010. Abundance and Chemical Speciation of Phosphorus in Sediments of the Mackenzie Delta, Chukchi Sea and Bering Sea: Importance of Detrital Apatite. Aquatic Geochemistry, 16: 353-371, doi: 10.1077/s10498-009-9081-4 (Abstract or pdf online).

Douglas, T.A., Fortier, D., Shur, Y.L., Kanevskiy, M.Z., Guo, L., Cai, Y. and Bray, M.T. 2011. Biogeochemical and geocryological characteristics of wedge and thermokarst-cave ice in the CRREL Permafrost Tunnel, Alaska. Permafrost and Periglacial Processes, 21(4), doi:10.1002/ppp709 (abstract).

• Ping, C.-L., G.J. Michaelson, L.D. Guo, M.T. Jorgenson, M. Kanevskiy, Y. Shur, F. Dou and J. Liang. 2011. Soil carbon and material flux across the eroding coastline of the Beaufort Sea, Alaska. JGR-Biogeosciences, doi:10.129/2010JG001588 (abstract or pdf file).

 

 

Export of old terrigenous organic matter across the arctic land/ocean interface: Evidence from estuarine sedimentary organic carbon 14C ages along the Siberian Arctic coast (from Guo et al., 2004, Global Biogeochemical Cycles, 18(1), GB1036, pdf file).

Increasing coastal erotion under a changing climate in the North

What are the impacts and biogeochemical consequencies of climate and environmental change in the north?

 

Interactions of Th(IV) with organic compound classes of marine organic matter (1999-2003)

A NSF Supported project (NSF-OCE #9906823):  Th-234 has been widely used as a tracer to quantify the fluxes of organic carbon export from the euphotic zone to the deep ocean in many global research projects, such as Joint Global Ocean Flux Study (JGOFS). Particulate organic carbon (POC) fluxes are calculated as the product of the 234Th export flux times the POC/234Th ratio. However, POC/234Th ratio in suspended and sinking particles are not constant. It varies with different particle sizes, water depths, and study areas. Understanding factors controlling the POC/234Th ratio in the ocean is of paramount importance in global carbon cycle study and thus the climate changes in the time scale of human concern.
The major objectives of the proposed work are: 1) To collect colloidal, suspended and sinking particles from marine environments for laboratory studies and to quantify the relationships between the abundance of polysaccharide enriched marine aggregates and Th(IV) deficiency (See photos of R/V Gyre 2000 and 2001Cruises); 2) To conduct controlled laboratory experiments to investigate the interactions of Th (IV) with individual organic compound classes of marine organic matter to elucidate whether complexing of Th is a non-selective physicochemical process (less likely) or organic ligand dependent (most likely); 3) To characterize the bulk chemical, surface chemical, and isotopic composition of marine organic matter to determine their acid-base and ligand properties and the nature of organic or inorganic functional groups that Th (IV) tracks; 4) To construct an improved scavenging model which relies on information on Th(IV) sorption to strong surface active and non-surface active ligand groups in particulate and colloidal organic matter. Experimental and model results will be used to recommend improvements in the use of POC/234Thp ratios for POC flux determinations.

Role of natural organic matter in governing the bioavailability of potentially toxic metals to estuarine bivalves (SeaGrant/NOAA, 2001-2004)

Supported by NOAA-Sea GrantBivalves have been extensively used as bio-indicator organisms in environmental assessment and monitoring programs to assess the bioavailable contaminant concentrations in coastal environments (e.g., NOAA's NS&T program). Natural dissolved organic matter (DOM) is ubiquitous and is a potentially nutritious food source for bivalves. However, the presence of DOM may significantly alter the bioavailability and biogeochemical cycling pathways of many trace metals in marine environments. The role of natural DOM in governing the bioavailability of potentially toxic metals to bivalves is not well understood and has rarely been tested. To better use bivalves as pollution indicator organisms, a thorough understanding of metal uptake pathways and mechanisms as a function of the quality and quantity of DOM is sorely needed. The primary objectives of this research is to determine how DOM affects the bioavailability of metals to bivalves, including oysters (Crasostrea virginica) and mussels (Ischadium recurvum), and whether DOM can be directly used as a food source by these bivalves using radiotracers and molecular probes in controlled laboratory experiments. Our research will provide crucial information applicable to environmental assessment and monitoring.
This is a research project supported by the NOAA-Sea Grant between 2001-2004, collaborating with Professor Peter Santschi and Professor Sammy Ray in the Laboratory for Oceanographic and Environmental Research (LOER) at the Texas A&M University at Galveston.   My previous SeaGrant supported project, namely "
Bioavailability of colloid-associated metals to estuarine bivalves" was completed during 1998-2001. 
Ssee our publications in Environmental Science and Technology (Guo et al. 2001, pdf) and Marine Environmental Research (Guo et al., 2002, pdf).
 


Nature and fluxes of nutrients and organic matter from Yukon River Basin (IARC, 2001-2004)
 

Principal investigator, Supported by Japan Frontier Research System for Global Change/IARC: The Yukon River is one of the largest rivers draining into the Arctic with an annual discharge of more than 200 billion m
3 of freshwater and ~60 million tons of suspended sediment, and contributes ~8% of the total freshwater input to the Arctic Ocean.  Recent evidence shows that the polar region and the northern ecosystem are quite sensitive to global and regional climate and environmental changes.  Therefore, environmental and climate changes in the northern arctic and thus increasing freshwater flow and organic matter inputs from the Yukon River Basin will have profound impacts on the ecosystem and biogeochemical cycles in the Bering Sea and Arctic Ocean.  However, due to its remoteness and the extreme weather conditions, the Yukon River Basin remains a pristine and understudied basin, which can, in turn, serve as a natural laboratory for arctic environmental change studies.  We seek to understand the nature and fluxes of nutrients (N, P, Si) and natural organic matter from the Yukon River, as well as hydrological and water chemistry for a better understanding of biogeochemical consequences of climate and resulting environmental changes in the north.  See our publications in Water Researh (Guo et al., 2003, pdf), Global Biogeochemical Cycles (Guo et al, 2004, pdf) and Biogeochemistry (Gueguen et al., 2005, pdf).


From Guo et al. (2004), Speciation and fluxes of nutrients (N, P, Si) from the upper Yukon River, Global Biogeochemical Cycles, 18(1), GB1038 (pdf).


Winter sampling from Chena River, Alaska

Radiocarbon and molecular characterization of organic compound classes of dissolved organic matter in the ocean

Dissolved organic carbon (DOC) is the largest organic C pool in the ocean and plays an important role in the global carbon cycling. However, understanding the cycling of DOC in the ocean remains a major challenge to oceanographers despite recent advances in marine organic carbon cycling. To further explore the detailed biogeochemical pathways of different organic components and to better understand the complex cycling of DOC in the ocean, measurements of isotopic composition in different organic compound classes are sorely needed, as are the chemical and molecular characterization of different size or molecular weight fractionated DOC components.
The major objectives of the proposed research are: 1) To collect large quantities of high molecular weight (HMW) dissolved organic matter from seawater for chemical and isotopic characterizations, using cross-flow ultrafiltration techniques; 2) To characterize elemental composition, major organic compound classes and individual organic molecules using elemental analysis, pyrolysis-GC/MS and liquid extraction techniques; 3) To determine radiocarbon (14C) and stable carbon (d13C) and nitrogen (d15N) isotope signatures of major organic compound classes, amino acids, carbohydrates, total lipids, and acid-insoluble fractions in isolated HMW DOC fractions; 4) To elucidate how chemical composition and isotope signatures of the compound classes reflect their production and decomposition processes in the ocean and to better understand the biogeochemical pathways and turnover times of each compound class, whose geochemical behavior could be significantly different from that of the total DOC pool.

This is a collaborative proposal declined by the National Science Foundation (NSF) - Ocean Science Division.

A schematic diagram of a ultrafiltration system for isolating colloids and naoparticles from natural waters (from Guo 1995).


Freeze dried colloidal organic matter samples from estuarine seawater


Atomic force microscopy image of aquatic colloids (from Santschi et al., 1998, L&O)

Research collaborators
 

Name

Department

Affiliation

Dr. Peter Santschi

Oceanography and Marine Sciences

Teaxs A&M University

Dr. Orjan Gustaffson

Institute of Applied Environmental Research

Stockholm University

Dr. Johan Ingri

Division of Applied Geology

Lulea University

Dr. Per Andersson

Laboratory for Isotope Geology

Swedish Museum of Natural History

Professor Yipu Huang

Department of Oceanography

Xiamen University

Dr. Min Chen

Department of Oceanography

Xiamen University

Dr. Igor Semiletov

 Pacific Oceanological Institute

Vladivostok, Russia

Dr. Wenxiong Wang

Department of Biology

Hong Kong Univ. of Sci. & Technology

 Dr. Dan White

 Water & Environ Research Center

 University of Alaska Fairbanks

 Dr. Xiaoling Ding

Environmental Sciences

 University of South Florida

Dr. Chien-Lu Ping

Soil Sciences
Soil organic chemistry and climate change

University of Alaska Fairbanks

Dr. Chin-Chang Hung 

Oceanography

Taiwan Ocean University

Dr. Robie Macdonald

Marine Environmental Quality

Institute of Ocean Science

Dr. Jamie Lead

Water Chemistry, GEES

University of Birmingham

Dr. Tom Douglas

Environmental Science

CRREL, Alaska

Dr. Ming-Yi SUN

Marine Science

University of George


Major Equipment

Total organic carbon and nitrogen analyzer (Shimadzu TOC-V) with capability of measuring DOC and TN;

Canberra gamma counting system, with ultrahigh purity Ge well detector and multichannel analyzer;

Shimadzu RF-5301 Spectroflurometer;

Aglient 8453 Spectrophotometer;

TIDAS-LWCC High Performance fiber optic spectrophotometer (World Precision Instruments)

Autoanalyzer  (Seal) for nutrient analysis;

Flow field-flow fractionation (FlFFF) and SPLITT systems (Postnova Analytics) which can be coupled with on-line detectors such as UV absorbance, fluoresce, refractive index, light scattering. and ICP-MS, providing continuum size spectra in colloidal and nanoparticle materials in natural waters.

Cross-flow ultrafiltration systems (e.g., Millipore Proflux M-30 and Amicon DC-10 systems);

Ultra-pure water system, ultracentrifuge, freeze dryer, clean benches, auto balances, etc.

See also Center for Trace Analysis at USM
 

CTD
CTD deployment during a research cruise to the Arctic Ocean aboard R/V Mirai (JAMSTEC).


Ultrafiltration system for processing large volume of seawater to isolate sufficient quantities of colloidal organic matter


 

Photos of 2001 R/V Gyre 04 Cruise

Photos of 2000 R/V Gyre 06 Cruise

Photos of 2001 R/V Mirai K-04 Cruise

 

 

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Last updated  Wednesday, June 01, 2011